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Variations in the effects of specific staphylococcal cell-wall peptides on endothelial and smooth-muscle cells.
The sequence of cell-wall peptides derived from the well characterized strain of Staphylococcus aureus 01/20 was recently determined (Klebba et al., 1988, Microbiology, 144, 471-480). In the present study, the effect of these peptides on endothelial and smooth-muscle cells was assessed. Peptides 0.1-10 micrograms/ml stimulated endothelial cells to produce the cytokines interleukin-1 and interleukin-6, whereas these peptides did not affect the production of the tissue inhibitor of metalloproteinases-1, a proteinase inhibitor synthesized by smooth-muscle cells. A dose-response curve was established, which shows that peptides 0.1-1.0 micrograms/ml stimulated endothelial cells to produce interleukin-1 and interleukin-6. Peptides 10-100 micrograms/ml stimulated endothelial cells to produce tissue inhibitor of metalloproteinases-1. The purified peptides were found to be noncytotoxic and nonsensitizing. These data suggest that these peptides exert their effects on endothelial cells through cytokine-mediated mechanisms, whereas the effects on smooth-muscle cells may be a result of alterations in the extracellular matrix.Ex-member of Bruder, vice-chairman and vice-president
Ex-Bruder, chairman, and vice-president of the editorial staff of the Austrian newspaper Die Presse, has added his voice to the debate over the fate of Austria’s only daily newspaper.
His opinion, the gist of which was read on Austrian Radio yesterday, is that if the war in the Balkans had not ended quickly enough, Die Presse would have ended up in the editorial offices of the Austrian National Council.
In the late 1990s, the media played a big part in convincing the public that Austria’s Armed Forces would ac619d1d87
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